Imaging & Microscopy
- Immunohistochemistry (IHC)
- Immunofluorescence staining (IF)
- Confocal laser scanning microscopy
Methods & Technologies
Our lab employs a broad spectrum of methods and technologies for cardiovascular basic research.
Protein Biochemistry & Interactomics
- Protein interaction studies (Co-IP, pull-down)
- Mass spectrometry
- Phosphoproteomics
Further methods
Cell Culture & Model Systems
- Primary culture of neonatal rat cardiomyocytes (NRVCM)
- Primary culture of adult rat cardiomyocytes (ARVCM)
- Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM)
- HEK293 cell lines
- E. coli expression systems
Molecular Biology & Cloning
- Quantitative real-time PCR (qRT-PCR)
- Advanced cloning (Gateway cloning system)
- Virus production (adenovirus)
- Adeno-associated virus (AAV) production
Electrophysiology
- Patch-clamp recordings in cardiomyocytes
In Vivo Phenotyping
- Cardiac echocardiography (mouse)
- Pressure-volume loop measurements (PV loop)
- Cardiac histology and histopathology